Mac and PC

I have an external drive connected to my mac loaded with my movies, shows, etc. that in turn is then viewed through my Panasonic tv using serviio. I now want to copy all my files onto a separate drive and give to friends who use a pc and have serviio loaded. Question is is it possible or will the pc not read those files that are copied from the mac. Serviio working very well I might add and happy with the service.