Hello again atc98092,  I had rather let this thread die as I was (and still do) struggle to understand both the technicalities of trans-coding video; the setting-up of Serviio;... and the black art that is "file sharing" under Vista! 
 It was revitalised just the other day by Emma's suggestion of "ByteCopy". but I must admit to having "shelved" the project since August, so I've kinda lost track of which files were produced by what software and what options were included, sound tracks/subtitles!
Thanks for your guidance on FileBot, I stumbled around trying to get some meaningful output from it when suddenly, there it was, the entire bibliography of all the episodes of each series of MASH...impressive, but I couldn't say how I accomplished it I'll have to re-visit it later! 
Similarly, I stumbled around the GUI for MakeMKV before achieving useful output. Your detail rings some bells from the attempts I made and will be a useful guide for me when I get time to try again, thankfully my options will be few as my disks are not Blu-Ray.
The state of play at the moment is this.
I have created a folder Named "MKV Output Files" which contains eleven files.
The first eight are ".mkv" files generically named "title00.mkv to title07.mkv:
Two further files are transcriptions of "title00" and "title01" named "MASH - The Pilot.ts" and "To Market, To Market.ts" respectively.
There is one more file in this folder named "MASH - The Pilot.mp4" which I suspect was trans-coded using "WinX Mobile Video Converter"
I have entered the filepath to the folder on the library tab of Serviio, and it shows up on my Sony TV under the folder name "MKV Output Files"; however, when selected I'm told "There are no items to display" despite the above files being in residence!
I have established (I think) that this seems to be a "sharing" problem, as I have copied and pasted into the folder, some files which Serviio can "see" elsewhere in the library, but the copies placed in this folder are not detected.
I have also copied ".ts" and ".mkv" files from the "MKV Output Files" folder, and pasted them into folders who's content 
is accessible to serviio, and though they can be seen (actual thumbnails are shown rather that generic placeholders) neither file will play, I just get the "Temporarily cannot access server" message... there is however another ".ts" file in the same folder which plays just fine, though I can't say what software created it!
Since the folder was already "Shared" with "Admin" as "Owner", I have now extended that to include "Everyone as Co-Owner", so the folder is now shared with the World and it's dog... but apparently still not with Serviio! Grrr!
The other odd thing is the fact that no matter how long I wait after clicking on "Force refresh" and "Show status"...the "checking for new files" indicator remains green, ad-infinitum with no notification of any found items!
Your suggestion to check that Folders are "Displayed" led me to a tab that I've never viewed before, but all the items listed have the "Displayed" status already.
Now 3am here so I need to get to bed!
You have re awakened my interest in this project, and I'll try to devote some time to it over the Christmas holidays, I'm grateful to you for taking the time to help me.
You have also brought back memories of my childhood watching "Joe Friday" in Dragnet!  
